

The AELAB Inverted Biological Microscope AE-IM400 is designed for advanced cell biology, tissue culture, and live specimen observation. With wide-field optics, phase contrast capability, and ergonomic design, it ensures accurate imaging while preserving sample integrity. A reliable choice for professional laboratories and research facilities.
An inverted biological microscope is a type of microscope where the light source and condenser are located above the stage, while the objectives are below. This design allows for the observation of living cells and organisms in culture dishes or flasks, without the need to disturb the samples.Inverted microscopes are particularly useful in cell biology, tissue culture, and other fields where specimens need to be observed in their natural state or in liquid environments. The unique configuration makes it easier to focus on specimens at the bottom of containers, providing clear images of cells and structures that might be difficult to view with a traditional upright microscope.
Features
The AE-IM400 positions the objectives beneath the specimen stage while the illumination and condenser sit above. This inverted configuration allows researchers to observe live cells at the bottom of culture dishes and flasks without disturbing them. Combined with infinity plan optics and optional phase contrast, the microscope delivers sharp, high-contrast images suitable for both routine and advanced biological studies.
Clear, wide-field imaging with 22mm eyepieces
Versatile objective system supporting brightfield & phase contrast
Ergonomic trinocular head for comfortable viewing & documentation
Large mechanical stage suitable for culture plates and flasks
Flexible illumination options including LED and fluorescence modules
Long working distance condenser for easy handling of tall culture vessels
Educational Labs: Standard AE-IM400 with halogen illumination and brightfield optics
Research Centers: AE-IM400 with phase contrast objectives and LED illumination
Pharmaceutical & QC Labs: AE-IM400 with epi-fluorescence attachment for cell viability studies
Biotech Startups: Compact AE-IM400 configurations with cost-efficient objectives
Cell culture observation
Tissue engineering studies
Live cell imaging
Food & pharmaceutical laboratories
University research departments
Quality control in biotech and chemical industries
Specifications :
| Item | Specification | Outfits | |
| Eyepiece | WF10×/22mm | ●● | |
| LWD Infinity Plan Objectives | LPlanFL 4×/0.11 | W.D.=12.1mm | ○ |
| LPlanFL 10×/0.25 | W.D.=10.3mm | ● | |
| LplanFL 20×/0.45 | W.D.=5.8mm | ● | |
| LplanFL 40×/0.65 | W.D.=5.1mm | ● | |
| LWD Infinity Plan Phase Contrast Objectives | LPlanFL
PHP 4×/0.10 |
W.D.=9.2mm |
○ |
| LPlanFL
PHP 10×/0.25 |
W.D.=10.3mm |
○ | |
| LPlanFL
PHP 20×/0.45 |
W.D.=5.8mm |
● | |
| LPlanFL
PHP 40×/0.65 |
W.D.=5.1mm |
○ | |
| Annular Spot | 10×/20×/ 40× | ● | |
| 4× | ○ | ||
| Seidentopf
Trinocular Head |
Inclined 45°, interpupilary distance: 48-76mm
Light distribution (both): 100: 0(100% for eyepiece) 80:20 (80% for trinocular head, and 20% for eyepiece) |
● | |
| Seidentopf
Trinocular Head |
Inclined 45°, interpupilary distance: 48-76mm
Light distribution (both): 100: 0 and 0:100 (100% for eyepiece or 100% for trinocular head) |
○ | |
| Nosepiece | Quintuple | ● | |
| Mechanical Stage | Stage size: X×Y: 210×241mm, Round slide size:Φ110mm,
Attached mechanical stage (available for 96 holes plate, moving range X×Y:128×80mm.) |
● | |
| Culture Dish Holder | 65mm | ○ | |
| 54mm | ● | ||
| 35mm | ○ | ||
| blood cell counter | ○ | ||
| Condenser | Long working distance, Quickly detachable , N.A.0.3,
Working distance: 72mm (with condenser),195mm(without condenser). |
● | |
| Koehler Illumination | 6V/30W (input voltage:100V~240V) | ● | |
| 5WLED,input voltage:100V~240V | ○ | ||
| Filter | Blue | ● | |
| Green | ● | ||
| Amber /Grey | ○ | ||
| C-mount | 1 X /0.5X /0.75X C-Mount (focus adjustable) | ○ | |
| Epi Fluorescence Illumination | Epi fluorescence media unit , field diaphragm,center adjustable. | ○ | |
| 100W mercury lamp ,5WLED(input voltage:100V~240V) | ○ | ||
| B, G, V, UV fluorescence filters can be chosen | ○ | ||
| Item | Specification | Outfits | |
| Eyepiece | WF10×/22mm | ●● | |
| LWD Infinity Plan Objectives | LPlanFL 4×/0.11 | W.D.=12.1mm | ○ |
| LPlanFL 10×/0.25 | W.D.=10.3mm | ● | |
| LplanFL 20×/0.45 | W.D.=5.8mm | ● | |
| LplanFL 40×/0.65 | W.D.=5.1mm | ● | |
| LWD Infinity Plan Phase Contrast Objectives | LPlanFL
PHP 4×/0.10 |
W.D.=9.2mm |
○ |
| LPlanFL
PHP 10×/0.25 |
W.D.=10.3mm |
○ | |
| LPlanFL
PHP 20×/0.45 |
W.D.=5.8mm |
● | |
| LPlanFL
PHP 40×/0.65 |
W.D.=5.1mm |
○ | |
| Annular Spot | 10×/20×/ 40× | ● | |
| 4× | ○ | ||
| Seidentopf
Trinocular Head |
Inclined 45°, interpupilary distance: 48-76mm
Light distribution (both): 100: 0(100% for eyepiece) 80:20 (80% for trinocular head, and 20% for eyepiece) |
● | |
| Seidentopf
Trinocular Head |
Inclined 45°, interpupilary distance: 48-76mm
Light distribution (both): 100: 0 and 0:100 (100% for eyepiece or 100% for trinocular head) |
○ | |
| Nosepiece | Quintuple | ● | |
| Mechanical Stage | Stage size: X×Y: 210×241mm, Round slide size:Φ110mm,
Attached mechanical stage (available for 96 holes plate, moving range X×Y:128×80mm.) |
● | |
| Culture Dish Holder | 65mm | ○ | |
| 54mm | ● | ||
| 35mm | ○ | ||
| blood cell counter | ○ | ||
| Condenser | Long working distance, Quickly detachable , N.A.0.3,
Working distance: 72mm (with condenser),195mm(without condenser). |
● | |
| Koehler Illumination | 6V/30W (input voltage:100V~240V) | ● | |
| 5WLED,input voltage:100V~240V | ○ | ||
| Filter | Blue | ● | |
| Green | ● | ||
| Amber /Grey | ○ | ||
| C-mount | 1 X /0.5X /0.75X C-Mount (focus adjustable) | ○ | |
| Epi Fluorescence Illumination | Epi fluorescence media unit , field diaphragm,center adjustable. | ○ | |
| 100W mercury lamp ,5WLED(input voltage:100V~240V) | ○ | ||
| B, G, V, UV fluorescence filters can be chosen | ○ | ||
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